منابع مشابه
Microtiter Immunocyto- chemical ELISA Assay
Quantitative assessment of immunoreactivity and protein expression in cultured cells can be time-consuming and subjective. The problem is magnified when rapid and efficient screening is required, and the supply of cells is limited. Immunoblot analysis requires protein extraction, quantification of protein content and gel electrophoresis, which make the method impractical for evaluating multiple...
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Biofilms are communities of microbes attached to surfaces, which can be found in medical, industrial and natural settings. In fact, life in a biofilm probably represents the predominate mode of growth for microbes in most environments. Mature biofilms have a few distinct characteristics. Biofilm microbes are typically surrounded by an extracellular matrix that provides structure and protection ...
متن کاملMicrotiter plate immunoenzymometric assay for estrogen receptor.
The estrogen receptor (ER) status of breast cancer is used both as a prognostic factor and as a predictor of response to endocrine therapy. An immunoenzymometric assay for ER was developed on 96-well microtiter plates (EIA96). This technique involves two monoclonal antibodies directed against different epitopes in the B domain of ER. The two-step protocol (16-18 h and 3 h at 4 degrees C) requir...
متن کاملNonradioactive fluorescence microtiter plate assay monitoring aptamer selections.
Benchmarks Since its discovery in 1990, aptamer technology (1,2) has emerged as a novel and increasingly important approach to evolve specifically binding nucleic acid molecules (3). As aptamers can be developed to a vast range of target molecules, their potential application is very diverse. They can be used as molecular recognition elements in analytical systems for detection, separation, or ...
متن کاملMicrotiter hemagglutination-inhibition assay for staphylococcal enterotoxin B.
The rapid microtiter technique was investigated as a means of facilitating the detection of staphylococcal enterotoxin B. With this technique, many samples were assayed simultaneously, and readable results were obtained in 3 hr. Other advantages of this method, in addition to speed, were the small quantity of reactants used, ease of reading, and reproducibility.
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ژورنال
عنوان ژورنال: BioTechniques
سال: 1999
ISSN: 0736-6205,1940-9818
DOI: 10.2144/99266bm15